Abstract—For a macroalga
Cladophora glomerata, the extracellular matrix on the surface of algae cell, extracellular polymeric substances (EPS) has a gel-like structure that could fractioned into loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS). The ratio of protein to polysaccharides (P/S) is TB-EPS is higher than LB-EPS. The contents and extraction rate of EPS were compared two chemical extraction methods (formaldehyde-NaOH and NH4OH). The analysis demonstrated that more extraction rates of EPS were achieved by the formaldehyde-NaOH method. The protein to polysaccharides ratio(P/S) in EPS is between 0.299 to 0.375 by formaldehyde-NaOH and between 0.232 to 0.374 by NH4OH. The results indicated that the EPS could be efficiently extracted by the formaldehyde-NaOH method, but the influence of P/S ratios were not obvious.
Index Terms—
Cladophora glomerata, algae, extracellular polymeric substances (EPS), protein, polysaccharide
The authors are with the Department of Environmental Engineering, Kun Shan University, No.195, Kunda Rd., YongKang Dist., Tainan City 710-03, Taiwan (e-mail: sheu@mail.ksu.edu.tw, spchang@mail.ksu.edu.tw, lyc007@mail.ksu.edu.tw).
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Cite: Hwey-Lin Sheu, Shui-Ping Chang, and Yi-Chao Lee, "Comparison of EPS Extraction Efficiences from Cladophora Glomerata by Two Chemical Extraction Methods," International Journal of Chemical Engineering and Applications vol. 10, no. 4, pp. 126-129, 2019.