Abstract—Apium graveolens Linn. is in Apiaceae family, the
common name is A. graveolens or celeric. Chemical
constituents of A. graveolens found sedanolide, celerin,
celephthalide and flavonoids compounds which are repellent
mosquito, antioxidant and antibacterial. This study used the
root of A. graveolens were extracted by methanol. The crude
methanol extracted isolated by the stationary phase of rice husk
ash (RHA). Modified the powders of RHA by sieve sizes of Mesh
20, 40 and 100 gave three particle size are 660, 382 and 143 μm
respectively, and cleaned by sodium hydroxide. The crude
methanol was isolated by each of the particle size of RHA gave
F1-F3 and were examined chemical constituents by thin layer
chromatography. The fungicides used Rhizopus nigricans is a
fungus commonly known as black bread. The orchid farms in
Thailand found R. nigricans damaged leaves and roots of
orchids. F1-F3 were tested by antifungal assay, as disc diffusion
dilution, turbidity cell density is measurement by %
transmitant at 625 nm by ultra-violet spectrometer and
minimum fungicidal concentration (MFC). F1-F3 were
inhibited fungicides in 68, 53 and 21 μg/ml, respectively. The
aimed of this study used RHA from the rice-bran oil industry
were absorbent for isolated the phytochemical compounds from
A. graveolens which have the anti-fungicidal activity.
Index Terms—Adsorbent, antifungal, celephthalide, rice
husk ash, sieves.
C. S. Department of chemistry, Faculty of Science and Technology,
Suansunandha Rajabhat University,Thailand (e-mail: chanyapat.sa@
ssru.ac.th).
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Cite: Chanyapat Sangsuwon, "Rice Husk Ash as an Adsorbent for Isolated the Celephthalides Compounds from Root of Apium Graveolens Linn for Inhibited Phytopathogenic Fungi in Plants," International Journal of Chemical Engineering
and Applications vol. 10, no. 5, pp. 134-137, 2019.
