General Information
    • ISSN: 2010-0221 (Print)
    • Frequency: Bimonthly
    • DOI: 10.18178/IJCEA
    • Editor-in-Chief: Prof. Dr. Shen-Ming Chen
    • Executive Editor: Jennifer X. Zeng
    • Abstracting/ Indexing: Chemical Abstracts Services (CAS), Ulrich's Periodicals Directory, CABI, Electronic Journals Library, Google Scholar, ProQuest,  Crossref, EBSCO.
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Prof. Dr. Shen-Ming Chen
National Taipei University of Technology, Taiwan

IJCEA 2014 Vol.5(1): 26-30 ISSN: 2010-0221
DOI: 10.7763/IJCEA.2014.V5.345

Decolorization of Synthetic Dyes by Crude Laccase from Lentinus Polychrous Lev.

Karnika Ratanapongleka and Jirapa Phetsom
Abstract—In the present study, the addition of copper (2mM) to the fungus L. polychrous cultivated in solid media influenced the laccase activity. Laccase production was increased in the presence of copper and reached a maximum activity of 145 U/ml on 12th day. Crude laccase was capable to decolorize different dye structures. The enzyme showed great decolorization efficiency toward Acid Blue 80 (85% decolorization in 120 min). Reaction kinetics of the enzyme on Acid Blue 80 followed Michaelis-Menten behavior and the initial rate of decolorization depended on the dye concentration. The kinetic parameters of the enzyme were determined and calculated from Lineweaver-Burk plots. The results indicated that the Km value was 0.36 mM and Vmax value was 0.0017 mM/min. The pH value for maximum decolorization of Acid Blue 80 was 5.0.

Index Terms—Decolorization, laccase, Lentinus polychrous.

K. Ratanapongleka is with the Chemical Engineering Department, Faculty of Engineering, Ubon Ratchathani University, Ubonratchathani 34190, Thailand (e-mail:
J. Phetsom is with the Biology Department, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150, Thailand (e-mail:


Cite: Karnika Ratanapongleka and Jirapa Phetsom, "Decolorization of Synthetic Dyes by Crude Laccase from Lentinus Polychrous Lev.," International Journal of Chemical Engineering and Applications vol. 5, no. 1, pp. 26-30, 2014.

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