Abstract—Plants responses to internal signals and environmental stimuli are regulated by a complex mechanism of signal transduction networks. Brassinosteroids (BRs) are important signal mediators which play significant role in plant development and defense responses to environmental stress. To explore the biological functions of BRs biosynthetic pathways, we conducted a systematic search for specific inhibitors of BRs biosynthesis. In the present work, we report the synthesis of a novel triazole derivative with coumarin moiety (YCZ-FL) as a fluorescent probe. The fluorescence properties and the biological activity of YCZ-FL on BRs biosynthesis inhibition were investigated. We found that YCZ-FL exhibits highly fluorescence intensity with a maximum excitation wavelength at 336 nm, and maximum emission wavelength at 442 nm, respectively. YCZ-FL inhibits stem elongation of Arabidopsis seedlings grown in the darkness with an IC50 approximately 1.56 ± 0.05 μM. To distinguish the primary site of actions on biosynthesis of GA and BRs, effect of co-application of GA (1 μM) and/or BL (10 nM) on YCZ-FL treated Arabidopsis seedlings were evaluated. We found that co-application of BL reversed the inhibition of stem elongation treated by while GA did not.
Index Terms—Brassinosteroid biosynthesis inhibitor, fluorescent labeling probe, plant growth regulators.
The authors are with the Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, 241-438, Shimoshinjo Nakano, Akita 010-0195, Japan (e-mail: jmwang@akita-pu.ac.jp).
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Cite: Tomoki Hoshi, Tadashi Matsumoto, Kazuhiro Yamada, Yuko Yoshizawa, and Keimei Oh, "Synthesis of New Brassinosteroid Biosynthesis Inhibitor with Coumarin Moiety as a Fluorescent Probe," International Journal of Chemical Engineering and Applications vol. 5, no. 5, pp. 379-383, 2014.
