Abstract—In this paper, I studied on the influence of some point mutations of green fluorescent protein (GFP) gene on expression efficiency in Escherichia coli (E. coli). It was found that some mutant genes of GFP had influenced on the expression efficiency of GFP gene. In this paper, I acquired six GFP mutants (A402G, A675C, A462G, A88G, T357C, A443G), compared the differences among the six mutant sites, and discussed the relationship between the mutant gene and protein expression efficiency. It was found that A443G had the lowest relative fluorescence intensity (0.04 fold), and A88G had the lower relative fluorescence intensity (0.16 fold), A402G had the higher relative fluorescence intensity (1.2 fold), against wildtype of GFP. I hope that the findings, which may be applicable to genetic engineering, will be helpful for further studies of protein expression.
Index Terms—Mutant gene, gene expression, green fluorescent protein, fluorescence intensity.
Xiaoliang Hao is with the Department of Life Science and Green Chemistry, Saitama Institute of Technology, 1690, Fusaiji, Fukaya, Saitama 369-0293, Japan and School of Chemical Engineering, University of Science and Technology LiaoNing, 185 Qianshan Middle Road, High-tech zone, Anshan, LiaoNing, 114501, China (e-mail: haoxiaoliang1980@163.com).
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Cite: Xiaoliang Hao, "Influence of Some Point Mutations of Green Fluorescent Protein Gene on Expression Efficiency in Escherichia coli," International Journal of Chemical Engineering and Applications vol. 7, no. 4, pp. 259-263, 2016.
