General Information
    • ISSN: 2010-0221
    • Frequency: Bimonthly
    • DOI: 10.18178/IJCEA
    • Editor-in-Chief: Dr. Eldin W. C. Lim
    • Executive Editor: Mr. Ron C. Wu
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Editor-in-chief
Dr. Eldin W. C. Lim
Dept. of Chemical and Biomolecular Engineering,
National University of Singapore, Singapore
IJCEA 2016 Vol.7(6): 369-372 ISSN: 2010-0221
doi: 10.18178/ijcea.2016.7.6.607

A Novel Polyclonal Rabbit Immunoglobulin G Crystallisation Approach Using 3D Nanotemplate

Kuan L. Tan, Peck L. Kiew, Nilanjon Naskar, and Jerry Y. Y. Heng
Abstract—Protein crystallisation is the formation process of protein crystal from a supersaturated solution in which metastable supersaturated solution containing the protein interested reaching a stable low energy state by reduction of solute concentration. In recent years, protein crystallisation has spurred the interest among researchers to explore its potential application as one of the downstream processing methods for low-cost production and purification of proteins. Heterogeneous template nucleant is the key factor for successful protein crystallisation. It reduces the energy barrier for nucleation by constrains the protein molecules and promotes the protein molecules to aggregate in crystalline order in order to facilitate the protein crystallisation. This report focuses on the identification of optimal crystallising conditions for polyclonal immunoglobulin G subclass 4 (IgG4) proteins by employing three-dimensional (3D) nanotemplates as the facilitating nucleant. The comparison of crystal growth of the polyclonal IgG4 was performed with and without the presence of 3D nanotemplate. The results revealed that mesoporous 3D nanotemplates were able to facilitate crystallisation process of polyclonal IgG4. The optimal crystallisation conditions for polyclonal IgG4 was successfully determined. Polyclonal IgG4 was found to crystallise optimally at a protein concentration of 10mg/ml using 3D nanotemplates with pore size 10nm, PEG 8000 as precipitant, Calcium Acetate and Imidazole as buffer at the incubation temperature and pH of 20oC and 7 respectively.

Index Terms—3D nanotemplate, crystallisation, IgG4, heterogeneous nucleant.

Kuan L. Tan and Peck L. Kiew are with UCSI University, Malaysia (e-mail: leonttkl@hotmail.com, kiewpl@ucsiuniversity.edu.my). Nilanjon Naskar is with Indian Institute of Technology Kharagpur, India (e-mail: cnilanjon.naskar15@imperial.ac.uk). Jerry Y. Y. Heng is with Imperial College London, UK (e-mail: jerry.heng@imperial.ac.uk).

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Cite: Kuan L. Tan, Peck L. Kiew, Nilanjon Naskar, and Jerry Y. Y. Heng, "A Novel Polyclonal Rabbit Immunoglobulin G Crystallisation Approach Using 3D Nanotemplate," International Journal of Chemical Engineering and Applications vol. 7, no. 6, pp. 369-372, 2016.

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