Abstract—A series of mesoionic pyrimidinium betaines were synthesized and tested in vitro for their antioxidant activity using three tests: 2,2-diphenyl-1-picrylhydrazil (DPPH) free radical scavenging, Ferric Reducing Antioxidant Power (FRAP) and bleaching of β-carotene. The results indicated that pyrimidinium betaines possess antioxidant properties with three methods. In the DPPH assay, the monocyclic pyrimidinium betaine showed the highest activity with an IC50 value of 300 μg/mL. The pyrimidinium betaines also revealed a reducing power lower than that of the control antioxidants, and the monocyclic pyrimidinium betaine was the most active one. In the β-carotene bleaching test, the oxidation of linoleic acid was inhibited by all the betaines. The bicyclic one showed the greatest inhibitory activity of the oxidation of linoleic acid with a relative antioxidant activity (RAA) of 90%, an activity higher than that of the reference antioxidant α-tocopherol (41%). It was found that the antioxidant efficacy increased with increasing concentration of betaine and depended on the nature of the substituent on the pyrimidine ring.
Index Terms—Antioxidant ativity, β-carotene bleaching, dpph assay, frap assay, pyrimidinium betaine.
F. Malki and A. Touati are with the Laboratoire de recherche sur les produits bioactifs et valorisation de la biomasse, Ecole Normale Supérieure, BP 92,16000, Kouba, Algiers, Algéria (e-mail: malki_fatiha76@yahoo.fr, a_touati2000@yahoo.fr).
S. Moulay is with the Laboratoire de Chimie-Physique Moléculaire et Macromoléculaire, Département de Chimie Industrielle, Faculté de TEchnologie, Université Saâd Dahlab de Blida, Blida, Algeria (e-mail: polymchemlab@hotmail.com).
Michel Baltas is with the Laboratoire de Synthèse et Physicochimie de Molècules d’Intèrêt Biologique, UMR 5068, Université Paul Sabatier, 118, route de Narbonne - 31062 Toulouse Cedex 9, France (e-mail: baltas@chimie.ups-tlse.fr).
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Cite: F. Malki, A. Touati, S. Moulay, and M. Baltas, "Evaluation of Antioxidant Activity of Some Mesoionic Pyrimidinium Betaines by Three Different Methods," International Journal of Chemical Engineering and Applications vol. 7, no. 6, pp. 373-377, 2016.
