General Information
    • ISSN: 2010-0221
    • Frequency: Bimonthly
    • DOI: 10.18178/IJCEA
    • Editor-in-Chief: Dr. Eldin W. C. Lim
    • Executive Editor: Mr. Ron C. Wu
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Editor-in-chief
Dr. Eldin W. C. Lim
Dept. of Chemical and Biomolecular Engineering,
National University of Singapore, Singapore
IJCEA 2016 Vol.7(6): 378-382 ISSN: 2010-0221
doi: 10.18178/ijcea.2016.7.6.609

The Development of Immobilization Matrices with Adjustable Density for Use in the Immobilization of Stationary-Phase Operating Microorganisms within Continuous Bioreactors

Robert W. M. Pott
Abstract—Stationary phase organisms can be entrapped in immobilization matrices to prevent their being ‘washed-out’ of a bioreactor. A consequence of immobilization is that these beads, or immobilization supports, tend to settle out of solution, since they are more dense than the surrounding media. To counter this more mixing energy must be added to the reactor in the form of agitation in order to keep the beads in suspension, and to prevent mass transfer limitations. One solution to this issue is to include low density glass microspheres within the immobilization matrix in order to bring the overall density of the matrix closer to that of the media, and so reduce mixing energy requirements. This article outlines experiments demonstrating the use of glass microspheres in the production of calcium alginate hydrogel beads (a commonly used immobilization matrix) and shows the effect their addition has on the circulation of beads within a thermosiphon as a test photobioreactor which has no direct mixing energy added, but approximately 0.5 mW of indirect mixing energy added through the absorbance of light. The demonstration of this methodology highlights a potentially industrially applicable method for immobilization-matrix modification, leading to better bioreactor volume utilization and lower mixing energy expenses.

Index Terms—Bioprocess engineering, cell immobilization, immobilization matrix, reactor design, continuous operation, thermosiphon.

R. W. M. Pott is with the Department of Process Engineering, University of Stellenbosch, South Africa (e-mail: rpott@sun.ac.za).

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Cite: Robert W. M. Pott, "The Development of Immobilization Matrices with Adjustable Density for Use in the Immobilization of Stationary-Phase Operating Microorganisms within Continuous Bioreactors," International Journal of Chemical Engineering and Applications vol. 7, no. 6, pp. 378-382, 2016.

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