Abstract—Twenty three isolates which were collected from soil samples around UTAR, Kampar, Perak and Pantai Bagan Nakhoda Omar, Sabak, Selangor were successfully isolated using chitinase selective medium supplemented with colloidal chitin and bromocresol purple as indicator. Based on their enzyme production, two most potent isolates were selected for identification. The isolates were tentatively identified as
Enterobacter sp. and
Zymomonas sp. based on their morphological and biochemical test. The preliminary study showed that both
Enterobacter sp. and
Zymomonas sp. produced the highest chitinase at 48 hours. The process optimization was carried out using one-factor-at-a-time and the parameters involved were the percentage of colloidal chitin, incubation temperature and pH. The optimum parameters for
Enterobacter sp. to produce chitinase were 5 % colloidal chitin, at 30 °C and pH 7 with 2.92 U/mL/day of productivity. As for
Zymomonas sp., the chitinase productivity (2.54 U/mL/day) was obtained from the optimum conditions i.e. 15 % colloidal chitin at 30 °C and pH 6.
Index Terms—Bromocresol purple, colloidal chitin, Enterobacter,
Zymomonas.
L. G. A. Ong is with the Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Perak, Malaysia (e-mail: ongga@ utar.edu.my).
H. K. Lam, M. Y. Lim, T. X. Tan were with the Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Perak, Malaysia.
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Cite: Lisa G. A. Ong, Hoi Khei Lam, Mei Yin Lim, and Tian Xiang Tan, "Process Optimization on Chitinase Production by Locally Isolated Enterobacter sp. and Zymomonas sp.," International Journal of Chemical Engineering and Applications vol. 8, no. 4, pp. 286-289, 2017.
