Abstract—The paper presents the results of modification of outer interfaces of oil drops of multiple emulsions W
1/O/W
2 for targeted drug delivery. The modification involves physical adsorption of antibodies: anti-CD15 (Hodgkin's disease marker). The effect of molecular interaction between molecules of antibody (protein) and oil phase compounds was measured using isothermal titration calorimetry (ITC). The ITC analysis also included investigation of interactions between components of emulsion and antibody buffer. Multiple emulsions were prepared by a one-step method, in a bioreactor with the Couette-Taylor flow. The inner phase of the emulsion was an aqueous solution of alginic acid, membrane phase was soybean oil, the outer phase was distilled water, appropriate surfactants were added to each phase. The influence of the structure of multiple emulsions (double emulsions with single internal drops and many internal drops) on adsorption of antibodies was investigated. The ITC analysis showed that antibodies interacted with emulsion compounds. The change in heat rates of molecular interactions suggested adhesion of protein onto oil drops interfaces.
Index Terms—Antibody adsorption, isothermal titration calorimetry, drug delivery systems, multiple emulsion.
A. Metera, E. Dluska, and A. Markowska-Radomska are with Warsaw University of Technology, Faculty of Chemical and Process Engineering, 00-645 Warsaw, Warynskiego 1, Poland (e-mail: agatametera@gmail.com, ewa.dluska@pw.edu.pl, Agnieszka.markowska@pw.edu.pl).
B. Tudek and K. Kosicki are with University of Warsaw, Faculty of Biology, Institute of Genetics and Biotechnology 02-096 Warsaw, I. Miecznikowa 1, Poland (e-mail: tudek@ibb.waw.pl, konrado@biol.uw.edu.pl).
T. Fraczyk is with the Institute of Biochemistry and Biophysics Polish Academy of Sciences, 02-106 Warszawa, Pawinskiego 5a, Poland (e-mail: fraczykt@gmail.com).
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Cite: A. Metera, E. Dluska, A. Markowska-Radomska, B. Tudek, T. Fraczyk, and K. Kosicki, "Functionalized Multiple Emulsions as Platforms for Targeted Drug Delivery," International Journal of Chemical Engineering and Applications vol. 8, no. 5, pp. 305-310, 2017.
