General Information
    • ISSN: 2010-0221
    • Frequency: Bimonthly
    • DOI: 10.18178/IJCEA
    • Editor-in-Chief: Prof. Dr. Shen-Ming Chen
    • Executive Editor: Mr. Ron C. Wu
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Editor-in-chief
Prof. Dr. Shen-Ming Chen
National Taipei University of Technology, Taiwan
 

IJCEA 2017 Vol.8(6): 361-366 ISSN: 2010-0221
doi: 10.18178/ijcea.2017.8.6.684

An Amperometric Biosensor Based on Fumarate Reductase for Enzymatic Properties and Electrical Behavior

Yemin Yu, Yonghong Hu, Tao Li, Wenge Yang, Yuhao zhang, and Renlun Deng
Abstract—In this paper, the production of fumarate reductase was studied. Through ultrasonic separation, gel chromatography and 65% ammonium sulfate fractionation, the fumarate reductase specific activity reached to 106 U/mg, the purification ratio was 12.8 times and the recovery was 24%. Fumarate reductase was fixed on electrode, which was modified polyethyleneimine reductive graphene composites, through covalent bonding and electrostatic adsorption. The electrode was used to replace coenzyme NADH in the cell inside metabolism, constructing a fumarate reductase catalytic system based on electrical driving.
     The results showed that fumaric acid reductase immobilized on the electrode not only maintained a certain biological activity, but also could catalyze the metabolism of fumaric acid. Thus, the electrical catalysis of fumarate reductase/polyethyleneimine reductive graphene composites/glassy carbon electrode and the substrate of fumaric acid based on fumarate reductase were studied, providing a scientific theoretical basis for the production of succinic acid in vitro.

Index Terms—Fumarate reductase, fumaric acid, succinic acid, electronical catalytic behavior.

The authors are with College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China (e-mail:1253753161@qq.com, yonghonghu11@126.com).

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Cite: Yemin Yu, Yonghong Hu, Tao Li, Wenge Yang, Yuhao zhang, and Renlun Deng, "An Amperometric Biosensor Based on Fumarate Reductase for Enzymatic Properties and Electrical Behavior," International Journal of Chemical Engineering and Applications vol. 8, no. 6, pp. 361-366, 2017.

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