Abstract—In this study, we evaluated the influence of the sample volume (10 μL and 20 μL) upon the injection to a developed capillary gel electrophoresis (CGE) system designed using a short-length capillary (total length of 15 cm and effective length of 7.5 cm). The fused silica capillary was coated with an acrylamide linear polymer chain to prevent the electroosmotic flow in the direction opposite to the movement of DNA samples. The sieving polymer solution used was hydroxyethyl cellulose with a molecular weight of 1,300,000. Using this CGE system for the separation of a 100-bp DNA Ladder sample, the sample injection volume had a substantial effect on the electropherogram results. The error bars for migration time, mobility, and resolution length were much larger when using 20 μL of the sample than using 10 μL. The use of 10 μL of the sample provided highly reliable results. These findings indicated that developers of CGE equipment should pay close attention to the sample injection volume in order to measure small DNA samples with high accuracy.
Index Terms—Capillary gel electrophoresis, injection, point-of-care testing, polymer solution.
Tomoka Nakazumi and Yusuke Hara were with Research Institute for Sustainable Chemistry, ISC, National Institute of Advanced Industrial Science and Technology, AIST, Central 5-2, 1-1-1 Higashi, Tsukuba 305-8565, Japan (e-mail: y-hara@ aist.go.jp).
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Cite: Tomoka Nakazumi and Yusuke Hara, "Influence of the Sample Volume upon Injection on Capillary Gel Electrophoresis for the Separation of Small DNA Fragments," International Journal of Chemical Engineering and Applications vol. 9, no. 1, pp. 16-19, 2018.
