Influence of the Sample Volume upon Injection on Capillary Gel Electrophoresis for the Separation of Small DNA Fragments - Volume 9 Number 1 (Feb. 2018) - IJCEA
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    • ISSN: 2010-0221
    • Frequency: Bimonthly
    • DOI: 10.18178/IJCEA
    • Editor-in-Chief: Prof. Dr. Shen-Ming Chen
    • Executive Editor: Mr. Ron C. Wu
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  • May 30, 2018 News! Vol.9, No.3 has been published with online version. 6 peer reviewed papers are published in this issue.   [Click]
  • Mar 02, 2018 News! Vol.9, No.1 has been published with online version. 6 peer reviewed papers are published in this issue.   [Click]
Editor-in-chief
Prof. Dr. Shen-Ming Chen
National Taipei University of Technology, Taiwan
 

IJCEA 2018 Vol.9(1): 16-19 ISSN: 2010-0221
doi: 10.18178/ijcea.2018.9.1.692

Influence of the Sample Volume upon Injection on Capillary Gel Electrophoresis for the Separation of Small DNA Fragments

Tomoka Nakazumi and Yusuke Hara
Abstract—In this study, we evaluated the influence of the sample volume (10 μL and 20 μL) upon the injection to a developed capillary gel electrophoresis (CGE) system designed using a short-length capillary (total length of 15 cm and effective length of 7.5 cm). The fused silica capillary was coated with an acrylamide linear polymer chain to prevent the electroosmotic flow in the direction opposite to the movement of DNA samples. The sieving polymer solution used was hydroxyethyl cellulose with a molecular weight of 1,300,000. Using this CGE system for the separation of a 100-bp DNA Ladder sample, the sample injection volume had a substantial effect on the electropherogram results. The error bars for migration time, mobility, and resolution length were much larger when using 20 μL of the sample than using 10 μL. The use of 10 μL of the sample provided highly reliable results. These findings indicated that developers of CGE equipment should pay close attention to the sample injection volume in order to measure small DNA samples with high accuracy.

Index Terms—Capillary gel electrophoresis, injection, point-of-care testing, polymer solution.

Tomoka Nakazumi and Yusuke Hara were with Research Institute for Sustainable Chemistry, ISC, National Institute of Advanced Industrial Science and Technology, AIST, Central 5-2, 1-1-1 Higashi, Tsukuba 305-8565, Japan (e-mail: y-hara@ aist.go.jp).

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Cite: Tomoka Nakazumi and Yusuke Hara, "Influence of the Sample Volume upon Injection on Capillary Gel Electrophoresis for the Separation of Small DNA Fragments," International Journal of Chemical Engineering and Applications vol. 9, no. 1, pp. 16-19, 2018.

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