Abstract—The present study aim at studying the histological effects of both aluminum phosphate (Alum) and calcium phosphate (CAP) nanoparticles adjuvant in parallel with their potentials as adjuvant and the related immune response to tetanus toxoid vaccine adsorbed on both of them. Ninety Swiss albino mice were used in the experiment (50% adults and 50% juveniles). Mice were immunized intramuscularly with 0.125 ml adjuvanted tetanus toxoid vaccine. For alum adjuvant study, 27 adult mice and 27 juvenile ones were injected with alum adjuvanted vaccine and sacrificed weekly as triplects for 9 weeks. For calcium phosphate adjuvant study, 15 adult mice and 15 juvenile ones were injected with calcium phosphate adjuvanted vaccine and sacrificed weekly as triplects for 5 weeks. The effect of alum and calcium phosphate nanoparticles adjuvants in enhancing the immune response of tetanus toxoid vaccine were monitored through measurement of antibody titer in sera of mice. The pathological effect of both adjuvants were monitored through histological study of liver, brain, kidney and injected muscle of sacrificed animals. Recorded data revealed that both adjuvanted vaccine caused histopathological changes in tissues of liver, kidney, brain and injected muscle. On the other hand alum adjuvanted tetanus toxoid vaccine was more potent and showed higher antibody level than CAP adjuvanted vaccines.
Index Terms—Aluminum phosphate, calcium phosphate, nanoparticles, adjuvant, histology, vaccine.
Aliaa M. Issa is with the Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt (e-mail: aliaai@yahoo.com).
Mohamed S. Salim and Aly F. Mohamed are with the Holding Company for Biological Products and Vaccines (VACSERA), Dokki, Giza.
Hamdallah Zidan is with the Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Egypt.
Abdel-Razik H. Farrag is with the Department of Pathology, Medical Division, National Research Center, Dokki, Cairo, Egypt.
[PDF]
Cite: Aliaa M. Issa, Mohamed S. Salim, Hamdallah Zidan, Aly F. Mohamed, and Abdel-Razik H. Farrag, "Evaluation of the Effects of Aluminum Phosphate and Calcium Phosphate Nanoparticles as Adjuvants in Vaccinated Mice," International Journal of Chemical Engineering and Applications vol. 5, no. 5, pp. 367-373, 2014.
