Abstract—Lignocellulose ethanol is significantly sustainable bio-fuel. It is an environmentally friendly. This work is to develop a hydrolysis process of pretreated lignocellulose to ethanol production from narrow leaved cattail as a biomass material. Response surface methodology (RSM) with a central composite design (CCD) was followed to optimize the enzymatic saccharification process in order to obtain high glucose yield. Three independent variables (cellulase, β-glucosidase and temperature) that operating condition, vary at CCD five levels (-α, -1, 0, +1, +α). The optimum values for the predicted variables for glucose and xylose released were: cellulase loading 17.5 FPU/g substrate, β- glucosidase loading 0 U/g substrate and hydrolysis temperature 55°C. Under these optimal conditions, glucose and xylose yield reached to 413.25 mg/ g substrate and 75.48 mg/g substrate, respectively. The results of a confirmation experiment under the optimum conditions agreed with model predictions.
Index Terms—Enzymatic saccharification, lignocellulose, RSM, narrow leaved cattail, glucose produced.
A. Sopajarn is with the Department of Agricultural Machinery Technology, Rajamangala University of Technology Srivijaya Rattaphum College, Rattaphum, Songkhla 90180, Thailand (e-mail: am_rarrisa@hotmail.com).
C. Sangwichien is with the Department of Chemical Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand (e-mail: chayanoot.s@psu.ac.th).
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Cite: Arrisa Sopajarn and Chayanoot Sangwichien, "Optimization of Enzymatic Saccharification of Alkali Pretreated Typha angustifolia for Glucose Production," International Journal of Chemical Engineering and Applications vol. 6, no. 4, pp. 232-236, 2015.
