General Information
    • ISSN: 2010-0221
    • Frequency: Bimonthly
    • DOI: 10.18178/IJCEA
    • Editor-in-Chief: Dr. Eldin W. C. Lim
    • Executive Editor: Mr. Ron C. Wu
    • Abstracting/ Indexing: Chemical Abstracts Services (CAS), Ulrich's Periodicals Directory, CABI, DOAJ, Electronic Journals Library, Google Scholar, Engineering & Technology Digital Library, ProQuest, and Crossref
    • E-mail: ijcea@ejournal.net
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Editor-in-chief
Dr. Eldin W. C. Lim
Dept. of Chemical and Biomolecular Engineering,
National University of Singapore, Singapore
IJCEA 2010 Vol.1(2): 191-198 ISSN: 2010-0221
DOI: 10.7763/IJCEA.2010.V1.33

Recovery of Biological Products in Aqueous Two Phase Systems

Karnika Ratanapongleka

Abstract—Recovering of biological products, such as enzymes, proteins, nucleic acids, amino acids and microorganisms, from the contaminants requires many steps, each removal of the contaminants, isolating of target product and purification of product closer to the demand. For the success of the large scale production, there is a need for improving process economic, efficient and delicate downstream technique enough to preserve the properties of bio-products. Aqueous two phase systems (ATPSs) have overcome these demands and emerged as a powerful method for the downstream processing of bio-products. This paper is intended to encourage a starting idea for beginners in using ATPSs technique with biological products. Factors contributing to the partition behavior of biomolecules between the two phases including polymer molecular weight and concentration, biomolecule size, surface charge, pH and temperature are presented.

Index Terms— Aqueous two phase systems (ATPSs), Biomolecule, Partitioning

[PDF]

Cite: Karnika Ratanapongleka, "Recovery of Biological Products in Aqueous Two Phase Systems,"  International
Journal of Chemical Engineering and Applications
vol. 1, no. 2, pp. 191-198, 2010.  

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