1. 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Bulaksumur, Indonesia
2. Department of Chemistry, Sekolah Tinggi Analis Kimia Cilegon, Banten, Indonesia
Email: respati@ugm.ac.id (R.T.S.)
*Corresponding author
Manuscript received April 11, 2024; revised May 8, 2024; accepted July 2, 2024; published October 24, 2024
Abstract—Based on ethnopharmacology, Tristaniopsis merguensis Griff. is one of the indigenous Indonesian plants from the genus Tristaniopsis, which has the potential for biological activity, including its role as an antibacterial agent. This research aims to analyze the antibacterial activity of the extracts of the Tristaniopsis merguensis Griff. leaves against Staphylococcus aureus ATCC 25923 using the disc diffusion method with streptomycin as positive control and methanol as negative control. The chemical compounds of T. merguensis leaves were extracted using n-hexane, ethyl acetate, and methanol through the multilevel maceration method at room temperature for 2 ×24 hours. The results showed that methanol extract has the highest yield 12.7% compared to n-hexane and ethyl acetate, which are 0.78% and 10.58%, respectively. Phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, and tannins in the leaf extract. Gas Chromatography/Mass Spectrometry (GC–MS) analysis of active extract confided presence of caryophilene, hexadecanoic acid, α-acarenol, 9-octadecanoic acid, ethyl iso-allocholate, seline-3,7(11)-diene, among many compounds. The results of the disc diffusion antibacterial test on the diameter of the inhibitory zone of the leaves extract showed that the ethyl acetate extract of the leaves had the greatest inhibition against the bacteria S. aureus at concentration of 20,000 ppm with the inhibition clear zone diameters of 9.7 mm, respectively. Based on this data, it can be concluded that the ethyl acetate of T. merguensis holds significant potential as a source of antibacterial compounds.
Keywords—T. merguensis, antibacterial, S.aureus,patoghenic bacteria
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Cite: Taufik Hidayat, Winarto Haryadi, Boima Situmeang, and Respati Tri Swasono, "Antibacterial Activity of the Leaves Extract of Tristaniopsis Merguensis Griff from the Indonesian Indigenous Plant against Staphylococcus Aureus ATCC 25923," International Journal of Chemical Engineering and Applications vol. 15, no. 2, pp. 51-54, 2024.
