IJCEA 2025 Vol.16(1): 36-40
doi: 10.18178/ijcea.2025.16.1.36-40
The Combination of Molecularly Imprinted Polymer and Aptamer for Electrochemical Detection of Cancer Antigen 15-3
Chutimon Akkapinyo1, Porntip Khownarumit2, and and Rungtiva P. Poo-arporn1,*
1. Faculty of Engineering, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
2. 2Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Email: Email: chutimon.akk@gmail.com (C.A.); porntip.tas@kmutt.ac.th (P.K.); rungtiva.pal@kmutt.ac.th (R.P.P.)
*Corresponding author
Manuscript received November 17, 2024; accepted February 21, 2025; published May 16, 2025
Abstract—Cancer antigen 15-3 (CA 15-3) is a crucial biomarker for breast cancer monitoring. Tracking of CA 15-3 levels can aid in assessing treatment effectiveness and detecting disease recurrence. Herein, Molecularly Imprinted Polymer (MIP) and aptamer as recognition elements were developed to perform a sandwich assay for the selective electrochemical detection of CA 15-3. The sensitivity of Screen-Printed Carbon Electrode (SPCE) was improved by incorporating Multi-Walled Carbon Nanotubes (MWCNTs). Subsequently, MIP was fabricated layer on MWCNTs/SPCE for the initial selective capture of CA 15-3. The signal probe, a CA 15-3-specific aptamer functionalized with a polydopamine-cadmium complex (PDA-Cd-Apt), was then proposed for secondary selective binding to the captured CA 15-3. Additionally, PDA-Cd-Apt enabled signal generation for electrochemical measurement. Square Wave Voltammetry (SWV) was used to measure electrochemical response of cadmium ions, which was proportional to CA 15-3 concentration. The developed sandwich assay offered a detection range from 5 to 30 U/mL, with a detection limit of 4.44 U/mL. This sensor was suitable for CA 15-3 detection, offering cost-effectiveness, high sensitivity, simple fabrication, and ease of use.
Keywords—cancer antigen 15-3, electrochemical sensor, molecularly imprinted polymer, polydopamine nanoparticles
[PDF]
Cite: Chutimon Akkapinyo, Porntip Khownarumit, and Rungtiva P. Poo-arporn, "The Combination of Molecularly Imprinted Polymer and Aptamer for Electrochemical Detection of Cancer Antigen 15-3," International Journal of Chemical Engineering and Applications vol. 16, no. 1, pp. 36-40, 2025.
