doi: 10.18178/ijcea.2025.16.1.36-40
The Combination of Molecularly Imprinted Polymer and Aptamer for Electrochemical Detection of Cancer Antigen 15-3
Chutimon Akkapinyo1
,
Porntip Khownarumit2 ,
Rungtiva P. Poo-arporn1,*
- 1Faculty of Engineering, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
- 22Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
- Manuscript receivedNovember 17, 2024
- acceptedFebruary 21, 2025
- publishedMay 16, 2025
Abstract
Cancer antigen 15-3 (CA 15-3) is a crucial biomarker for breast cancer monitoring. Tracking of CA 15-3 levels can aid in assessing treatment effectiveness and detecting disease recurrence. Herein, Molecularly Imprinted Polymer (MIP) and aptamer as recognition elements were developed to perform a sandwich assay for the selective electrochemical detection of CA 15-3. The sensitivity of Screen-Printed Carbon Electrode (SPCE) was improved by incorporating Multi-Walled Carbon Nanotubes (MWCNTs). Subsequently, MIP was fabricated layer on MWCNTs/SPCE for the initial selective capture of CA 15-3. The signal probe, a CA 15-3-specific aptamer functionalized with a polydopamine-cadmium complex (PDA-Cd-Apt), was then proposed for secondary selective binding to the captured CA 15-3. Additionally, PDA-Cd-Apt enabled signal generation for electrochemical measurement. Square Wave Voltammetry (SWV) was used to measure electrochemical response of cadmium ions, which was proportional to CA 15-3 concentration. The developed sandwich assay offered a detection range from 5 to 30 U/mL, with a detection limit of 4.44 U/mL. This sensor was suitable for CA 15-3 detection, offering cost-effectiveness, high sensitivity, simple fabrication, and ease of use.
Keywords
- cancer antigen 15-3
- electrochemical sensor
- molecularly imprinted polymer
- polydopamine nanoparticles
How to Cite
Chutimon Akkapinyo, Porntip Khownarumit, and Rungtiva P. Poo-arporn, "The Combination of Molecularly Imprinted Polymer and Aptamer for Electrochemical Detection of Cancer Antigen 15-3," International Journal of Chemical Engineering and Applications, vol. 16, no. 1, pp. 36-40, 2025. https://doi.org/10.18178/ijcea.2025.16.1.36-40
Copyright & License
Copyright © 2025 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
